Effects of Oxygen Nanobubble Technology (NBsO2) on Hatching Rate and Larval Survival in Silver Barb Barbonymus gonionotus (Bleeker, 1849)

Rizka Maslikhatul Ulfa; Gatra Ervi  Jayanti; Husain Latuconsina

doi:10.52046/agrikan.v19i2.2837

Effects of Oxygen Nanobubble Technology (NBsO2) on Hatching Rate and Larval Survival in Silver Barb Barbonymus gonionotus (Bleeker, 1849)

Authors

Rizka Maslikhatul Ulfa Universitas Islam Malang, Indonesia
Gatra Ervi Jayanti Universitas Islam Malang, Indonesia
Husain Latuconsina Universitas Islam Malang, Indonesia

DOI:

https://doi.org/10.52046/agrikan.v19i2.2837

Keywords:

NBsO2, Ikan Tawes, Kualitas Air, Penetasan Telur, Sintasan Larva

Abstract

Oxygen nanobubble (O₂NB) technology can enhance dissolved oxygen availability in aquaculture systems. This study evaluated the effects of O₂NB application on the hatching rate and survival of silver barb (Barbonymus gonionotus) larvae and examined their relationships with water quality parameters. The experiment followed a completely randomized design with five O₂NB exposure durations (0, 5, 10, 15, and 20 min), each with five replicates. The measured parameters included hatching rate (HR), larval survival rate (SR), temperature, pH, and dissolved oxygen (DO). Data were analyzed using descriptive statistics and normality tests, followed by analysis of variance (ANOVA), the Kruskal–Wallis test, and paired-sample t-tests, as appropriate. O₂NB application had no significant effect on HR or SR (P > 0.05). Descriptively, the highest HR and SR were recorded in the 15-min treatment, at 29% and 4%, respectively. O₂NB application increased DO levels following treatment; however, the relationships between water quality parameters and HR or SR were not statistically significant (P > 0.05). These findings indicate that O₂NB application did not significantly affect egg hatching or larval survival under the experimental conditions evaluated.

Author Biographies

Rizka Maslikhatul Ulfa, Universitas Islam Malang

Program Studi Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Islam Malang, Jalan Raya Mayjen Haryono No. 193, Dinoyo, Lowokwaru, Kota Malang 65144, Jawa Timur, Indonesia

Gatra Ervi Jayanti , Universitas Islam Malang

Program Studi Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Islam Malang, Malang, Indonesia.

Husain Latuconsina, Universitas Islam Malang

Program Studi Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Islam Malang, Jalan Raya Mayjen Haryono No. 193, Dinoyo, Lowokwaru, Kota Malang 65144, Jawa Timur, Indonesia

References

Alam, H. S., Sutikno, P., Soelaiman, T. A. F., & Sugiarto, A. T. (2022). Bulk nanobubbles: Generation using a two-chamber swirling flow nozzle and long-term stability in water. Journal of Flow Chemistry. https://doi.org/10.1007/s41981-021-00208-8

Assersohn, K., Marshall, A. F., Morland, F., Brekke, P., & Hemmings, N. (2021). Why Do Eggs Fail? Causes Of Hatching Failure In Threatened Populations And Consequences For Conservation. Animal Conservation, 24(4), 540–551. https://doi.org/10.1111/acv.12674

Atoillah, I. (2016). Pengaruh Suhu Media Penetasan Yang Berbeda Terhadap Daya Tetas Telur Dan Kelulus hidupan Larva Ikan Wader Cakul (Puntius Binotatus). Diss. Universitas Brawijaya.

Cahyanti, W., Mumpuni, F. S., Maulidie, S. H., & Arifin, O. Z. (2022). Perkembangan Embrio dan Performa Awal Larva Ikan Tawes (Barbonymus gonionotus). Jurnal Mina Sains, 8(2). https://doi.org/10.30997/jmss.v8i2.7020

Diana, F., & Safutra, E. (2018). Pengaruh pemberian pakan alami yang berbeda pada benih ikan tawes (Barbonymus gonionotus) terhadap pertumbuhan dan kelangsungan hidup. Jurnal Akuakultura Universitas Teuku Umar, 2(1). https://doi.org/10.35308/ja.v2i1.769

Diansyah, S., Erina, Y., & Jannah, M. R. (2017). Pemberian pakan alami yang berbeda terhadap pertumbuhan dan kelangsungan hidup larva ikan nilem (Osteochilus hasseltii). Jurnal Akuakultura Universitas Teuku Umar, 1(1). https://doi.org/10.35308/ja.v1i1.478

Fuadi, A., Sami, M., Usman, U., & Saifuddin, S. (2020). Teknologi tepat guna budidaya ikan lele dalam kolam terpal metode bioflok dilengakapi aerasi nano buble oksigen. Jurnal Vokasi, 4(1), 39-45. https://doi.org/10.30811/vokasi.v4i1.1819

Gusrina, (2018). Genetika dan Reproduksi Ikan. Deepublish. Yogyakarta.

Haser, T.F., Febri, S.P., & Nurdin, M.S. (2018). Effectivity of the papaya leaf ’s extract in supporting hatching succes of the milk fish (Chanos chanos Forskall). Jurnal Agroqua: Media Informasi Agronomi dan Budidaya Perairan, 16(2), 92-99. https://doi.org/10.32663/ja.v16i2.427

Hutagalung, J., Alawi, H., & Sukendi, S. (2016). Pengaruh suhu dan oksigen terhadap penetasan telur dan kelulushidupan awal larva ikan pawas (Osteochilus hasselti CV) (Doctoral dissertation, Riau University).

Hutagalung, J., Alawi, H., Sukendi. (2017). Pengaruh Suhu dan Oksigen Terhadap Penetasan Telur dan Kelulushidupan Awal Larva Ikan Pawas (Osteochilus hasselti C.V.). JOM, 4(1):1–12. https://jom.unri.ac.id/index.php/JOMFAPERIKA/article/view/13196

Jayanti, G. E., Rahayu, T., Nasir, M. W., Rofida, N. V., & Dairobi, I. (2026). Nanobubbles of oxygen enhance water quality and increase the survival rate of Oreochromis niloticus fish. In BIO Web of Conferences (Vol. 209, p. 01012). 10.1051/bioconf/202620901012

Latuconsina, H. (2020). Ekologi Perairan Tropis: Biodeversitas, Adaptasi, Ancaman, dan Pengelolaannya. Yogyakarta: Gadjah Mada University Press.

Mauladani, S., Rahmawati, A. I., Absirin, M. F.,Saputra, R. N., Pratama, A. F., Hidayatullah, A., Dwiarto, A., Syarif, A., Junaedi, H., & Cahyadi, D. (2020). Economic feasibility study of Litopenaeus vannamei shrimp farming: Oxygen nanobubble investment in increasing harvest productivity. Jurnal Akuakultur Indonesia,19(1), 30–38. https://doi.org/10.19027/jai.19.1.30-38

Murhadisah. (2020). Efektivitas Ekstrak Daun Kunyit (Curcuma domestica) pada Penetasan Telur Ikan Bandeng (Chanos chanos Forskal). Jurnal Ilmiah Program Studi Perairan, 2(1): 62-72. https://doi.org/10.51179/jipsbp.v2i1.344

Muslim, I., & Atjo, A. A. (2021). Respon Penetasan Telur Ikan Mas (Cyprinus carpio) pada Tingkatan Suhu yang Berbeda. Siganus: Journal of Fisheries and Marine Science, 2(2), 147-153. https://doi.org/10.31605/siganus.v2i2.1017

Naskuroh, N., Tarsim, & Hudaidah, S. (2018), “Pengaruh Suhu yang Berbeda terhadap Daya Tetas dan Perkembangan Embrio Ikan Tawes (Barbonymus gonionotus)”, Jurnal Sains Teknologi Akuakultur, 2(2), 1–10.

Panggabean T.K, Sasanti A.D, Yulisman. (2016). Kualitas air, kelangsungan hidup, pertumbuhan, dan efisiensi pakan ikan nila yang diberi pupuk hayati cair pada air media pemeliharaan. Tesis Sarjana. Universitas Sriwijaya.

Pramleonita, M., Yuliani, N., Arizal, R., & Wardoyo,S. E. (2018). Parameter fisika dan kimia air kolam ikan nila hitam (Oreochromis niloticus). Jurnal Sains Natural, 8(1): 24-34. https://doi.org/10.31938/jsn.v8i1.107

Pratama, B. A., Susilowati, T., & Yuniarti, T. (2018). Pengaruh perbedaan suhu terhadap lama penetasan telur, daya tetas telur, kelulushidupan dan pertumbuhan benih ikan gurame (Osphronemus gouramy). Sains Akuakultur Tropis, 2(1), 59–65. https://doi.org/10.24198/sat.v2i1.2478

Rahayu, T., G. E. Jayanti, and A. Hayati (2023). “Induksi Nanobubbles (NBs) untuk Pertumbuhan Anggrek Dendrobium Imelda Marina Masagung X Bumi Menangis,” Metamorfosa: Journal of Biological Sciences, vol. 10, no. 1, p. 126.

Rahmawati AI, Saputra RN, Hidayatullah A, Dwiarto A, Junaedi H, Cahyadi D, Saputra HKH, Prabowo W, Kartamiharja UKA, Shafira H. (2021). Enhancement of Penaeus vannamei shrimp growth using nanobubble in indoor raceway pond. Aquaculture and Fisheries 6:277 -282. https://doi.org/10.1016/j.aaf.2020.03.005

Renita, R. (2017). Pengaruh suhu terhadap waktu penetasan, daya tetas telur dan kelangsungan hidup larva ikan cupang (Betta splendens) (Doctoral dissertation, Universitas Muhammadiyah Pontianak.

Scabra, A. R., & Setyowati, D. N. A. (2019). Peningkatan mutu kualitas air untuk pembudidaya ikan air tawar di Desa Gegerung, Kabupaten Lombok Barat. Jurnal Abdi Insani, 6(2), 267-275. https://doi.org/10.29303/abdiinsani.v6i2.243

Sinjai, H. 2011. Pengaruh Substrat Ijuk dan Hydrilla sp. terhadap Derajat Pembuahan dan Penetasan Telur Ikan Mas. Jurnal Perikanan dan Kelautan Tropis. 7 (1): 32-35. https://doi.org/10.35800/jpkt.7.1.2011.12

Song, W., Wang, C., Li, R., & Mu, C. (2017). Peroxiredoxin 6 From Cuttlefish (Sepiella Maindroni): Molecular Characterisation During Development And The Response To Thermal Stress. International Aquatic Research, 9(4), 281–292.

Tekile, A., Kim, I., & Lee, J.-Y. (2016). Extent and persistence of dissolved oxygen enhancement using nanobubbles. Environmental Engineering Research, 21(4), 427–435. https://doi.org/10.4491/eer.2016.028

Telaumbanua, M. (2025). Pengaruh oksigen terlarut (DO) dalam budidaya perairan. Jurnal Perikanan dan Kelautan, 2(1), 200-206. https://doi.org/10.70134/peraut.v1i1.691

Ulatowski, K., P. Sobieszuk, A. Mroz, T. Ciah, (2019). Stability of Nanobubbles Generated in Water Using Porous Membrane System. Chemical Engineering & Processing: Process Intensification 136, 62-71. https://doi.org/10.1016/j.cep.2018.12.010

Wahyuni, S., Nugroho, R. A., & Subandiyono, S. (2023). Pengaruh Larutan Daun Sirih (Piper betle) terhadap Daya Tetas Telur Ikan Mas (Cyprinus carpio). Sains Akuakultur Tropis: Indonesian Journal of Tropical Aquaculture, 8(1), 23–34.

Wei, Y., Ma, A., Deng, Y., Cao, M., Hu, Y., Cheng, Z., & Zhu, L. (2024). Effects of flow turbulence on the entire development process of drifting fish eggs. Fishes, 9(3), 88. https://doi.org/10.3390/fishes9030088

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Published

01-10-2026

How to Cite

Ulfa, R. M., Jayanti , G. E. ., & Latuconsina, H. (2026). Effects of Oxygen Nanobubble Technology (NBsO2) on Hatching Rate and Larval Survival in Silver Barb Barbonymus gonionotus (Bleeker, 1849). Agrikan Jurnal Agribisnis Perikanan, 19(2), 1–8. https://doi.org/10.52046/agrikan.v19i2.2837