Effect of Blood Cockle Anadara granosa on Spermatozoa Quality and Quantity of Black Tiger Shrimp Penaeus monodon
DOI:
https://doi.org/10.52046/agrikan.v18i2.2691Keywords:
Black Tiger Shrimp, Blood Cockle, Spermatozoa, Reproductive Performance, Spermatozoa AbnormalityAbstract
One of the primary factors contributing to the low natural mating rates of Penaeus monodon broodstock in ponds and hatcheries is the poor quality of male spermatozoa. This study aims to determine the optimal concentration of blood cockles (Anadara granosa) for improving the quality and quantity of spermatozoa in P. monodon. The experimental feeding treatments included 100% pellet, combination feed (50% blood cockle and 50% pellet), and 100% blood cockle. A total of 27 male broodstocks were used in this study, which lasted 30 days to assess the effects of the different feeding. Spermatophores were collected from the base of the fifth swimming leg, and spermatozoa were extracted, diluted in physiological NaCl solution, and counted using a hemocytometer. Spermatozoa density and abnormality percentage were analyzed using ANOVA, while spermatophore weight was analyzed using the Kruskal-Wallis test. The color and morphology of spermatophore were evaluated descriptively. The results indicated that a 100% blood cockle diet was the most effective in enhancing the quality and quantity of spermatozoa. Broodstock fed the 100% blood cockle showed the highest spermatozoa density (83.75 × 106 cells/mL) and the lowest abnormality percentage (25.00%). In comparison, the control group had a spermatozoa density of 37.03 × 106 cells/mL with 36.05% abnormalities, and the 50% blood cockle + 50% pellet group showed 49.69 × 106 cells/ml and 28.70% abnormalities. These findings demonstrate that at a 100% concentration of blood cockle, significantly improve the quantity and quality of spermatozoa in P. monodon.
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Copyright (c) 2025 Muhammad Fatratullah Muhsin, Andi Aliah Hidayani, Marlina Achmad , Dody Dharmawan Trijuno, Rosmiati Rosmiati, Besse Tenri Nurkamilah, Nunun Ainun Putri Sari Banun Kaliky, Latifa Baharuddin
This work is licensed under a Creative Commons Attribution 4.0 International License.
This work is licensed under a Creative Commons Attribution 4.0 International License.
