Antibacterial Activity of Piper betle Linn Extract Against Vibrio parahaemolyticus Causes of Disease in Vaname Shrimp (Litopenaeus vannamei)
DOI:
https://doi.org/10.52046/agrikan.v17i1.2072Keywords:
Piper betle Linn, Aktivitas Antibakteri, Vibrio parahaemolyticus, Litopenaeus vannamei, MIC dan MBCAbstract
Betel leaf (Piper betle L.) is a medicinal plant that has antibacterial properties. Betel leaves contain various chemical ingredients, including essential oils, phenols, terpenoids, tannins, polyphenols and steroids. This study aims to determine the inhibitory power of betel leaf extract (Piper betle L) on the growth of Vibrio parahaemolyticus which causes disease in vaname shrimp (Litopenaeus vannamei) and determine the minimum concentration of betel leaf extract that can inhibit growth and the minimum concentration that can kill V. parahaemolyticus internally. vitro. This research was carried out at the Integrated Biology Laboratory, Faculty of Fisheries and Marine Sciences, Indonesian Muslim University. A total of 500 g of betel leaf was extracted using the maceration method to produce 39.90 g of thick extract. Next, various concentrations of betel leaf extract were tested for their antibacterial effect on the growth of V. parahaemolyticus using the double layer method. The results of this research show that betel leaf extract is able to inhibit the growth of the bacteria V.parahaemolyticus. The minimum inhibitor concentration (MIC) value is at a concentration of 0.78 mg/mL and the maximum bactericidal concentration (MBC) at a concentration of 1.56 mg/mL, so it can be concluded that sirh leaf extract (Piper betle L) has the potential to be developed as an antibacterial for V. parahaemolyticus.
References
Anggia, M., Mutiar, S., & Arziah, D. (2018). Teknologi Ekstraksi Bunga Kenanga (Cananga Odorata L.) dan Sereh Wangi (Cymbopogon Nardus L. ) Sebagai Aroma Terapi Sabun Cair. Jurnal Daur Lingkungan, 1(1), 5. https://doi.org/10.33087/daurling.v1i1.2
Badan Pusat Statistik. (2017). Ekspor Udang Menurut Negara Tujuan Utama 2000- 2015.
Cordell, A. . (1981). Introdutcion to Alkaloids. John Wiley And Sons Inc.
De Schryver, P., Defoirdt, T., & Sorgeloos, P. (2014). Early Mortality Syndrome Outbreaks: A Microbial Management Issue in Shrimp Farming? PLoS Pathogens, 10(4), 10–11. https://doi.org/10.1371/journal.ppat.1003919
Edward, B. (2001). Clinical Relevance of Minimum Inhibitory Concentration(MIC). Aquaculture, 19, 289–296.
Feliatra, Zainuri, D., & Yoswaty. (2012). Pathogenitas Bakteri Vibrio sp. Terhadap Udang Windu (Penaeus monodon). Jurnal Sungkai, 2(1), 23–36.
Greenwood, D. (1995). Antibiotics Susceptibility (Sensitivity) Test, Antimicrobial and Chemotheraphy. Mc Graw Hill Company.
Hatmanti, A. (2003). Penyakit Bakterial pada Budidaya Krustasea serta Cara Penanganannya. J. Oseana, 28(3), 1–10.
Isnansetyo, A., & Kamei, Y. (2003). MC21-A, a Bactericidal Antibiotic Produced by a New Marine Bacterium, Pseudoalteromonas phenolica sp. nov. O-BC30 T , against Methicillin-Resistant Staphylococcus aureus. Antimicrobial Agents and Chemotherapy, 47(2), 480–488. https://doi.org/10.1128/AAC.47.2.480-488.2003
Kartasapoetra, G. (1992). Budidaya Tanaman Berkhasiat Obat. PT. Rineka Cipta.
Maulina, I., Handaka, A. A., & Riyantini, I. (2012). Analisis Prospek Budidaya Tambak Udang di Kabupaten Garut. Jurnak Akuatika.
Perschuere, L., Rombaut, G., Sorgeloos, P., & Verstraete, W. (2000). Probiotics Bacteria as Biocontrol Agents in Aquaculture. App. Environ. Microbiol, 64, 655–671.
Prescott, L. ., Harley, J. ., & Klein, D. A. (2005). Microbiology, Ed Ke-6. Mc- Graw-Hill.
Purwanto, S. (2015). Uji Aktivitas Antibakteri Fraksi Aktif Ekstrak Daun Senggani (Melastoma malabathricum L) terhadap Escherichia coli. Jurnal Keperawatan Sriwijaya, 2(2), 84–92.
Samirana, P. O., Swastini, D. A., Ardinata, I. P. R., & Suarka, I. P. S. D. (2017). Efek Ekstrak Daun Sirih Hijau (Piper Betle L.) Terhadap Pertumbuhan Bakteri Staphylococcus aureus. Jurnal Farmasi Udayana, 23.
Saraswati, D. (2011). Pengaruh Konsentrasi Ekstrak Daun Sirih Terhadap Daya Hambat Escherchia coli. Jurnal Healt & Sport, 3(2), 285–362.
Sastroamidjojo, S. (1997). Obat Asli Indonesia. Dian Rakyat.
Sheikh, M., Abdullah, R. M., Meghavanshi, M. K., & Irshad, M. (2012). Studies on Some Plant Extract for Their Antimicrobial Potential Against Certain Pathogenic Microorganisms. American Journal of Plant Sciences, 3, 209–213.
Suliantari. (2009). Aktivitas Antibakteri dan Mekanisme Penghambatan Ekstrak Sirih Hijau (Piper betle Linn) Terhadap Bakteri Patogen Pangan. Institut Pertanian Bogor. Bogor.
Susanty, S., & Bachmid, F. (2016). Perbandingan Metode Ekstraksi Maserasi Dan Refluks Terhadap Kadar Fenolik Dari Ekstrak Tongkol Jagung (Zea mays L.). Jurnal Konversi, 5(2), 87. https://doi.org/10.24853/konversi.5.2.87-92
Toar, A. I., Posangi, J., & Wowor, V. (2013). Daya hambat obat kumur Cetylpyridinium Chloride dan obat kumur daun sirih terhadap pertumbuhan Streptococcus mutans. JURNAL BIOMEDIK, 5(1).
Widowati, R. (2008). Keberadaan Bakteri Vibrio parahaemolitycus pada Udang yang dijual di Rumah Makan Kawasan Pantai Pangandaran. VIT VITALIS, 1(1).
Wijaya, H., Novitasari, & Jubaidah, S. (2018). Perbandingan Metode Ekstraksi Terhadap Rendemen Ekstrak Daun Rambui Laut (Sonneratia caseolaris L. Engl). Jurnal Ilmiah Manuntung, 4(1), 79–83.
Downloads
Published
How to Cite
Issue
Section
Citation Check
License
Copyright (c) 2024 Harlina Harlina, Azizah Ibrahim, Andi Hamdillah, Ilmiah Ilmiah

This work is licensed under a Creative Commons Attribution 4.0 International License.

This work is licensed under a Creative Commons Attribution 4.0 International License.







